The company faced a deadline Friday - lodge the appeal or stop its drivers from working in one of the largest cities in the world.
Londoners can still hail an Uber, after appeal documents have been filed.
Uber has appealed the decision to deny it a new operating license in London.
Uber's appeal is expected to take place on December 11.
The Financial Times reports that an Uber representative stated the appeal had been submitted while "constructive discussions" with TfL continue.
The legal battle follows purported peace talks between Dara Khosrowshahi, Uber's new chief executive, and TfL commissioner Mike Brown.
Khosrowshahi also published an open letter in London newspaper The Evening Standard apologising for his company's prior misdemeanours.
Uber's licence expired on 30 September after it was told by TfL told it that it would not be renewed over concerns that the ride-hailing firm is not "fit and proper" to operate a private hire service in London, citing concerns with the "conduct and approach" of the firm.
Mayor Sadiq Khan support TfL's decision and said: "All companies in London must play by the rules, if you do play by the rules, you're welcome in London".
The move comes as no surprise to anyone - Uber has 3.5 million punters registered in the United Kingdom capital, and it will desperately want to hang on to that custom - but what it means for users and drivers is that Uber carries on in London as normal for now. But this week, one MP grilling Uber's United Kingdom policy chief described the company as a "hypocrite", given that it is also appealing against driver rights in a separate legal battle.
Uber's United Kingdom policy chief Andrew Byrne responded: "I don't know, I think we are conscious of the fact that 40,000 people who do use Uber to make money in London".
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