Bezos is the largest shareholder of Amazon, the world's biggest online retailer.
Amazon's share price, and therefore Bezos' net worth, has jumped significantly since September.
Amazon shares rose 6.6 percent in 2017 and the company secured 89 percent of the online US spending market this holiday season among dominant stores. He first crossed the $100 billion net worth mark in November at the start of the holiday shopping season, once again helped by stock performance of Amazon. If adjusted for inflation, that cumulative total would now be over $144 billion in 2018.
According to a Bloomberg analysis, Gates' publicly disclosed donations show he has donated 700 million shares of Microsoft, worth $61.8 billion at today's value, along with $2.9 billion in cash. He lost the title later in the day when Amazon's stock dropped, returning him to second place with a net worth just below $90 billion.
Bezos started the year as the fourth wealthiest person before surpassing Buffett and business tycoon Amancio Ortega, Bloomberg reported in July.
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