It said: "I support LeBron James. He's doing an wonderful job for his community", the Chicago Bulls legend said.
In response, Mr Trump questioned James' intelligence, saying it was not easy to make the National Basketball Association player "look smart".
Saturday Trump faced criticism by a number of athletes who came to James's defense, including former United Kingdom and U of L basketball stars.
Many tweeters suggested that Trump may have held out from criticising James until he moved away from OH, which is a crucial swing state for the President.
This week, LeBron James opened The I Promise School, a fully-funded public elementary school catering to 240 at-risk teenagers in Akron, Ohio.
James, for his part, in his first comment since the insult, tweeted a celebration of his new school instead of responding directly to Trump.
"I can't sit back and say nothing", James said in his CNN interview.
This post Michael Jordan Defends LeBron James After Trump Twitter Attack first appeared on Vibe.
A statement from the first lady said James is doing "good things on behalf of our next generation", and compared his work to her childhood welfare initiative, "Be Best". Trump's former rival Hillary Clinton backed James as a "great family man" and "incredible ballplayer" who "gives back to his community, and isn't afraid to speak his mind".
Mr Trump has repeatedly been questioned about his views on race, from deeming many Mexican immigrants "rapists" during his first campaign speech for the Republican presidential nomination, to placing blame on "both sides" for the violent clash between white supremacists and counter-protesters in Charlottesville, Virginia, a year ago.
- China hits back at USA with $60bn tariff threat
- 2 women charged in Brooklyn nail salon brawl
- Barack and Michelle Obama start hiring for their Netflix project
- 'The Bachelorette': The most excruciating moments from a deeply uncomfortable finale
- Liverpool wrap up impressive pre-season with win against Torino
- Troy Balderson vs. Danny O'Connor
- Ruby Rose Cast As Batwoman for CW's Arrowverse
- Rick Gates returns to witness stand to detail Manafort’s finances
- Baby's body pulled from water near Brooklyn Bridge
- The Highest-Paid FC Barcelona Stars After Arturo Vidal Arrival