Authorities in Las Vegas issued a warrant for Cristiano Ronaldo's DNA on Thursday in order to see if it matches that on the dress of a woman who accused him of rape, according to the Wall Street Journal.
Ms Mayorga has given consent through her lawyers to make her name public.
Mayorga, now 34, reported to police that she'd been raped and received a medical examination.
Mayorga went public with her story in an interview with Der Spiegel in September, saying the Portuguese global raped her in a Las Vegas hotel room after a chance meeting in June 2009.
A law enforcement official with knowledge of the case told the Wall Street Journal that authorities in Sin City recently sent the warrant to court officials in Italy, where the 33-year-old now plays for Juventus.
Las Vegas police spokesperson Laura Meltzer confirmed than an official request has been submitted to Italian authorities.
The lawsuit is on and the criminal police investigation are on separate legal tracks.
Ronaldo and his lawyers repeatedly denied the claims when they first emerged in October, and at one point his lawyers threatened to sue the German news magazine that was the first to publish Mayorga's accusations. Recalling the interaction, Mayorga told Der Spiegel that Ronaldo had made a remark suggesting he was a good person minus that "1 percent".
Mayorga filed a lawsuit against the 33-year-old football superstar last year, saying she remained traumatized by her alleged ordeal and that she had been emboldened to take the step by the MeToo movement. "I have information that I believe will be beneficial to your case and I would like to help you".
Peter Christiansen, an attorney for Ronaldo, declined to comment Wednesday regarding the Las Vegas warrant.
"My clear conscious will thereby allow me to await with tranquillity the results of any and all investigations".
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